LOCUS       40924_12765        4707 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway conversion vector pCON-Cm-rare2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Kimura T, Nakao A, Murata S, Kobayashi Y, Tanaka Y, Shibahara K, 
            Kawazu T, Nakagawa T.
  TITLE     Development of the gateway recycling cloning system for multiple 
            linking of expression cassettes in a defined order, and direction on
            gateway compatible binary vectors
  JOURNAL   Biosci. Biotechnol. Biochem. 77 (2), 430-434 (2013)
  PUBMED    23391940
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Kimura T, Shibahara K, Tanaka Y, Nakao A, Kawazu T, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 4707)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4707)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2013"; volume: "77"; issue: "2"; 
            pages: "430-434"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pDONR201.
FEATURES             Location/Qualifiers
     source          1..4707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     652..668
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    676..800
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    807..830
                     /label=rare cutter sites
                     /note="rare cutter sites"
     promoter        853..883
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             937..1593
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    complement(1955..2079)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2580..2679)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2802..3608
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3754..4342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4477..4504)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4596..4682)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtatagaaaa
      121 gtaggctgga tgaatcgaat aacttcttct tcaaatgcgc aaacgaagaa atttcctcag
      181 tctcagccac agccgccgga gaaaaatgag aaaaaactga aaagggagag atttccggtg
      241 aaccctttga gagatttcag tacaagaagc tctggaagca gcagttgtag caatgagagt
      301 gcttctggtt caacttccgg tgaagcatcc aacggttgtc acaggttctt gctctctcat
      361 tctttctcat cgtcttcttc atcatcttct tcttcgttgg gagtttttcc tcgtagacca
      421 gtgagatcag tagctaaaaa ccccaaatct gctccggtag ttagtaaacc cttaattaga
      481 aagaaaccaa gctctcttga ggaggttaag ttgaaatcta ctcttactga gaaacctaat
      541 ttgctgaagt ctcagagatg caagacaaat cctgtatctg gaaagagacc aacttgtaag
      601 attaccatga aaccggagaa agtttcggtt ttgaagaaac aatcttctgt ttctagaact
      661 agtggatccc ccatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
      721 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
      781 caacatatcc agtcactatg gcggccggcg cgccgtttaa acttaattaa ggccgcatta
      841 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
      901 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
      961 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1021 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1081 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1141 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1201 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1261 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1321 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1381 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     1441 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     1501 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     1561 caacagtact gcgatgagtg gcagggcggg gcgtaatcgc gtggatccgg cttactaaaa
     1621 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     1681 atgtaaacgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     1741 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     1801 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     1861 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     1921 cccttataca cagccagtct gcaggtcgat cgaccatagt gactggatat gttgtgtttt
     1981 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     2041 attttacgtt tctcgttcag ctttcttgta caaagtggtg atatcaagct tcgacaatta
     2101 ttttggactc tcatgttctc aagctgaaat ccaaaagaaa catgatgttg gttcagattt
     2161 ggaatcactg acaatgaagt ttcagtcagc cggtttgtca ccaaggatcc aggcttcatc
     2221 atgggagccg agccgttcaa gtttcaactt cgactatttg gccacatctt ctgattctat
     2281 tgatctctca cagtttcaga gaggtttagt tgacaggagt tcttgtcatc ctcatgtaac
     2341 actggataaa gtgtcaagga cacatttgag agtagagcaa acaaatagcc acatgcctga
     2401 aatgaaatcc caacagatta cagatactga atttgatatt caaaatcaca aagaaagtgc
     2461 tgcttctctg ggaaaaggaa gagaattgct tccatgctct gctgcagagt caataagcac
     2521 tgatggaggt ggtctgattt gttctgagga ttcaaattgg atggcatgtt acaagaatta
     2581 cccaacttta ttatacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2641 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc
     2701 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2761 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     2821 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2881 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2941 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     3001 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     3061 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     3121 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     3181 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     3241 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     3301 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     3361 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     3421 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     3481 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     3541 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3601 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     3661 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3721 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3781 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3841 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3901 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3961 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4021 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4081 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4141 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4201 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4261 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4321 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4381 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4441 tattaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     4501 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     4561 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     4621 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
     4681 tgatgcctgg cagttcccta ctctcgc
//
